山梨醇麦康凯琼脂基础||SMAC |艾科试剂

称取本品55g，加入蒸馏水或去离子水1L，搅拌加热煮沸至完全溶解，分装三角瓶。勿高压灭菌，冷至50℃，每200毫升培养基加入0.05%无菌亚碲酸钾溶液(029220)1ml，如需增强对杂菌的抑制作用，可添加0.01mg头孢克肟至200毫升培养基中，混匀并倾注灭菌培养皿。Suspend 55 g in 1 L of distilled or deionized water.Heat with frequent agitation and boil to completely dissolve the powder.Distribute into flasks.Do not autoclave.Cool to 50℃.Add 1 ml of 0.05% sterile potassiun tellurite solution(029220)to 200 ml of the medium.If stronger inhibition to other bacteria is desired,add 0.01 mg cefixime to 200 ml of the medium.Mix thoroughly and pour into sterile Petridishes.

质量标准

蛋白胨Peptone.............................17g
月示胨Proteose peptone....................3g
猪胆盐Bile salts..........................5g
氯化钠Sodium chloride.....................5g
琼脂Agar..................................15g
D-山梨醇D-Sorbitol........................10g
结晶紫Crystal violet......................0.001g
中性红Neutral red.........................0.025g
最终pH Final pH...........................7.2±0.2

有效期：三年 Period of validity:Three years.

用途

用于致病性大肠杆菌(包括O157：H7)的分离培养。For islating and differenting pathogenic Escherchia coil(O 157:H7).

危险性质
贮存

旋紧瓶盖，贮存于避光、阴凉干燥处。Keep container tightly closed,store in a cool,dry place,away from bright light.

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