胰酪胨大豆酵母浸膏肉汤||Listeria Enrichment Broth Bas|艾科试剂

称取本品36g，加入蒸馏水或去离子水1 L，搅拌加热煮沸至完全溶解，分装三角瓶，121℃灭菌15min。称取0.1g 萘啶酮酸(029992)溶于10ml0.05mol/L氢氧化钠溶液中，另称取0.1g吖啶黄素(029990)溶于10ml灭菌蒸馏水或去离子水中，过滤除菌。使用前225ml培养基中加入萘啶酮酸溶液0.90ml和吖啶黄溶液0.34ml可制成EB增菌液。Suspend 36 g in 1 L of distilled or deionized water.Heat with frequent agitation and boil to completely dissolve the powder.Distribute into flasks.Autoclave at 121℃ for 15 minutes.Suspend 0.1g of nalidixic acid in 10 ml of 0.05mol/L.Sodium hydroxide solution and suspend 0.1g of acriflavine HCI in 10ml of sterile distilled or deionized water,stirelize by membrane filtration. To prepare the Listeria Enrichment Broth,add 0.90ml of nalidixic acid solution and 0.34ml acriflavine HCI solution to 225ml of the base medium.

质量标准

胰胨Tryptone..............................17g
多价胨Polypeptone.........................3g
酵母膏粉Yeast extract.....................6g
氯化钠Sodium chloride.....................5g
磷酸氢二钾Dipotassim hydrogen phosphate...2.5g
葡萄糖Dextrose............................2.5g
最终pH Final pH...........................7.3±0.2

用途

广泛用于细菌的培养，特别用于食品中李斯特氏菌的增菌培养。For cultivating bacteria,especially for enriching Listeriaspp in food.

危险性质
贮存

旋紧瓶盖，贮存于避光、阴凉干燥处。Keep container tightly closed,store in a cool,dry place,away from bright light.

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