牛津琼脂基础||OXA|艾科试剂

称取本品56.5g，加入1 L蒸馏水或去离子水，搅拌加热煮沸至完全溶解，分装三角瓶，121℃灭菌15分钟。冷至50℃，将放线菌酮0.4 g、多粘菌素E 0.02g、盐酸吖啶黄0.005g、头孢双硫唑甲氧0.002g、磷霉素0.01g溶于5ml乙醇和5ml水中制成混合溶液并过滤除菌, 每100 ml培养基中加入1ml混合溶液，摇匀后倾注灭菌培养皿。Suspend 56.5g in 1 L of distilled or deionized water.Heat with frequent agitation and boil to completely dissolve the powder.Distribute into flasks.Autoclave at 121℃ for 15minutes and then cool to 50℃.Suspend 0.4g of cycloheximide,and 0.02g of colistin sulfater,and 0.01g of acriflavine HCI,and 0.002g of cefotetan,and 0.01g of fosfomycin in 5ml ethanol and 5ml distilled by filter,add 1ml of the mix solution to 100ml of the base medium.Mix thoroughly and pour into sterile Petri dishes.

质量标准

多价胨Polypeptone.........................19g
可溶性淀粉Solube starch...................1g
氯化钠Sodium chloride.....................5g
琼脂Agar..................................15g
七叶苷Esculin.............................1g
柠檬酸铁铵Ferric ammonium citrate.........0.5g
氯化锂Lithium Chloride....................15g
最终pH Final pH...........................7.0±0.2

有效期：三年 Period of validity:Three years.

用途

用于单核增生李斯特氏菌的选择性分离。For selective isolation of Listeria monocytogenes

危险性质
贮存

旋紧瓶盖，贮存于避光、阴凉干燥处。Keep container tightly closed,store in a cool,dry place,away from bright light.

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