脑－心浸萃液态培养基|||艾科试剂

称取本品36g，加入1 L蒸馏水或去离子水，(如配成脑心浸萃琼脂培养基，另加入15g琼脂)，搅拌加热煮沸至完全溶解，分装试管或三角瓶，121℃灭菌15分钟。将40 ml 10%无菌牛胆汁加入60 ml脑—心浸萃液态培养基中，可配成胆汁液态培养基。Suspend 36g in 1 L of distilled or deionized water.(To prepare the Brain Heart Infusion Agar,add 15g agar to the medium.)Heart with frequent agitation and boil to completely dissolve the powder.Distribute into tubes or flaks.Autoclave at 121℃ for 15minutes.To prepare the medium containing oxgall,add 40ml 10% sterile oxgall solution to 60ml of the liquid medium.

质量标准

幼牛脑(提取浸出粉)Calf brains,infusion from...200g
牛心(提取浸出粉)Beefheart,infusion from...250g
葡萄糖Dextrose............................2g
氯化钠Sodium chloride.....................5g
月示胨Proteose peptone....................10g
磷酸氢二钠Disodium hydrogen phosphate.....2.5g
最终pH Final pH...........................7.4±0.2

用途

广泛用于霉菌、酵母、细菌的培养，包括营养要求较高的微生物的培养，特别用于城市供水中粪性链球菌滤膜法的确证实验。For cultivating moulds,yeasts and bacteria,include fastidious microorganism,especially for confirming the presence of Faecal streptococcus in urban water supply by membrane filtration.

危险性质
贮存

旋紧瓶盖，贮存于避光、阴凉干燥处。

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