三糖铁琼脂培养基（药典）||三糖铁培养基；TSI 琼脂|艾科试剂

粉红色粉末。一种鉴别培养基。由牛肉膏、酵母膏、蛋白胨、胨、葡萄糖、乳糖、蔗糖、硫酸亚铁、氯化钠、硫代硫酸钠、酚红、琼脂等配制而成。易吸湿。加水煮沸溶化后，成橘红色半透明液体，pH约7.5，冷至45℃开始凝固。
称取本品63.4g，加入蒸馏水或去离子水1L，搅拌加热煮沸至完全溶解，分装试管，121℃高压灭菌15min，放置高层斜面。Suspend 63.4g in 1L of distilled or deionized water.Heat with frequent agitation and boil to completely dissolve the powder.Distribute into tubes.Autocalave at 121℃ for 15minutes.Cool in slanted position with deep butts.

质量标准

胨Peptone.................................20.0g
牛肉浸出粉Beef extract....................5.0g
乳糖Lactose...............................10.0g
蔗糖Sucrose...............................10.0g
葡萄糖Dextrose............................1.0g
氯化钠Sodium chloride.....................5.0g
硫酸亚铁Ferrous sulfate...................0.2g
硫代硫酸钠Sodium thiosulfate..............0.2g
琼脂Agar..................................12g
酚磺酞Phenol red..........................0.025g
最终pH Final pH...........................7.3±0.1

用途

用于鉴别革兰氏阴性肠道菌发酵蔗糖、乳糖、葡萄糖及产生硫化氢的生化反应。For differentiating Gram-negavative enteric bacilli based on fermentation of sucrose,lactose and dextrose,and on hydrogen sulfide production.

危险性质
贮存

旋紧瓶盖，贮存于避光、阴凉干燥处。Keep container tightly closed,store in a cool,dry place,away from bright light.

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